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Practical Enzymology is written by Hans Bisswanger and published by John Wiley & Sons. It's available with International Standard Book Number or ISBN identification 3527820809 (ISBN 10) and 9783527820801 (ISBN 13).
A practice-oriented guide to assaying more than 100 of the most important enzymes, complete with the theoretical background and specific protocols for immediate use in the biochemical laboratory. Now expanded with a new section on metal ion determination.